DECODRTM

Biocomputing made easy and powerful

Accurate. Intuitive. Visual. CRISPR analysis at its full potential.

The Future of Gene Editing Starts Here

DECODRTM (Deconvolution of Complex DNA Repair) provides a convenient and user-friendly method to quantify the edits present in CRISPR-edited Sanger sequencing data.

DECODRTM is capable of analyzing a wide range of edits, including:

  • Indels (both single and multi-guide)
  • Multi-guide fragment excisions
  • Compound indels (combined insertion and deletion events)
  • HDR insertions and single-nucleotide substitutions

Using a computationally efficient algorithm, DECODRTM has virtually no limit on indel size (can quantify deletions several hundred bp long) and can determine the identity of inserted bases. DECODRTM supports batch analysis, in which several sequencing files can be analyzed at a time.

To use DECODRTM, all you need to do is perform standard Sanger sequencing reactions on your experimental files. The resulting .ab1 files can be uploaded to DECODRTM, and after providing either a text or .ab1 file for the reference sequence, simply submit your files for analysis to receive near NGS-level clarity in your output. For more information on DECODRTM, see our paper in The CRISPR Journal.

DECODRTM is available free of charge for all non-profit and academic use on the LatchBio platform. It is also available for licensing by for-profit institutions.

Need more information?

To find out more, email us at geneeditinginstitute@christianacare.org or call
302-623-5306.